In this paper, novel composite packaging films containing nano-particles—lanthanum doped nano-TiO2(La3+/nano-TiO2/lanthanum doped nano-ZnO (La3+/nano-ZnO) were prepared by solution pouring method. The XRD, TEM and LPSA were used to characterize the morphologies of nanoparticles. Then the barrier property, antibacterial performance and preservation performance of the films were also investigated. The lattice distortion that occurred in lattice of nano-TiO2 and nano-ZnO led the particle size reduced, surface energy increased and dispersity improved, which was caused by lanthanum doping. It was revealed that water vapor permeability and oxygen permeability of the composite films were decreased by the addition of nano-particles and the optimal barrier performances were obtained when mass fraction of nano-particles reached 1.2%. Antibacterial rates of the composite films against E. coli and S. aureus were both beyond 99%. Meanwhile, composite films showed efficient preservation performance on reducing over-ripening and aging of fruits and fungus. The strawberries were well preserved for 11 days at room temperature; the rotted rate and the weight loss rate were both controlled below 5%. Shelf life of agaricus blazeis was also extended from 2 days to 5 days at room temperature, due to the novel composite films slowed down the browning, autolysis and other phenomena during the storage of agaricus blazeis.